Primer

Part:BBa_K496002:Design

Designed by: Laurynas VANAGAS   Group: iGEM10_HokkaidoU_Japan   (2010-08-25)


Reverse primer for visualizing restriction enzyme digestion.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed after pSB1C3 pSB1A3 pSB1T3 plasmid universal primer sites.


Source

Restriction enzyme digestion cut offs must be big enough to view under UV light. When 2 restriction enzymes are used prefix and suffix cut offs must be distinguishable. Tried to make primer sites universal for every plasmid.

References